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Related post: 00030-15 00035-08 00036-18 00037-16 00040-09 00147-08 00148-08 00223-02 00224-02 00225-02 00226-02 00227-02 00228-02 00229-02 00259-02 00349-01 Antigen Recognition and Activation of Immuno- competent Cells. Paul Specificity in Immune Responses. Inman Ig Genetics: Ontogeny and Differentiation of Cells of the Rabbit Immune System. Mage Immunogenetics of Mouse Immunoglobulins and Genetic Control of Antibody Response. Lieberman Genetic Control of Immunocompetent Cell Inter- actions. Shevach The Mechanism of Activation of Thymus-Derived Lymphocytes. Schwartz Lymphocyte Interactions, Receptors and Functions. Green Cellular Interactions in the Immune Response. Schwartz Monoclonal Antibodies as Probes for T Cell Activa- tion. Shevach The Effects of UV irradiation on IL-1 Production by Keratinocytes and macrophages. Green Rabbit Allotypes: Structure, Organization and Regulated Expression of Ig Genes. Mage Biochemistry of Lymphocyte Activation. Waxdal Membrane Associated Glyconjugates and Glycoconjugate Receptors of Lymphoid Cells. Waxdal Studies on Lymphocyte Differentiation. Davis la Molecules and Immune Response Genes. Paul Structure and Function of Murine Class II MHC Genes and Gene Products. Germain Page 20- 1 20-15 20-16 20-17 20-18 20-19 20-20 20-21 20-22 20-23 20-24 20-25 20-26 20-27 20-28 20-29 20-30 PHS-NIH Summary Statement Office of the Chief Laboratory of Immunology October 1, 1982 through September 30, 1983 Introduction The Laboratory of Immunology is concerned with the elucidation of the fundamental mechanisms underlying immunologic responses. It has made rapid progress through the use of three new technologies which are creating a revolution in immunologic sciences. These are the use of monoclonal antibodies, the adaptation of techniques of moleular genetics to immunologic problems, and the use of long-term lines of cloned normal and transformed lymphocytes. The continued use and major improvement of these approaches should allow solution of many of the major problems which have concerned immunologists and should provide important approaches to the more precise regulation of the normal and the disordered immune response. An X- linked gene family expressed in lymphocytes . Laboratory of Immunology scientists have developed an approach to Buy Furoxone the creation of complementary DNA probes that recognize genes uniquely expressed in the messenger RNA of one but not another type of lymphocyte. For example, B cell specific cDNA probes have been produced by exhaustive hybridization of B cell cDNA with an excess of messenger RNA prepared from a T cell tumor. This B cell specific cDNA hybridizes to approximately 90% with messenger RNA derived from a B lymphoma. By contrast, the B cell specific cDNA will hybridize to less than 10% with messenger RNA from a T lymphoma. This approach may also be used to create cDNA cloned libraries of B cell specific and T cell specific genes. When a cDNA specific for T helper, but not T suppressor, cells was used to probe a "T cell specific" cDNA library, a limited number of clones were identified. Amongst these was one which upon Southern blotting proved Furazolidone Furoxone to recognize Furoxone Tablets a large number of distinct restriction fragments. By hybridizing this cDNA probe to the DNA of somatic cell hybrids which contained only a limited number of mouse chromosomes, it was shown that
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